Protein A Sepharose from Abcam

Product Specs
ItemProtein A Sepharose
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab193256
Quantity1 ML, 5 ML, 25 ML, 100 ML
Product TypeResins
ModeAntibody Purification
Sample TypeCell culture supernatant, Serum, Cell culture media, Ascites Fluid
ApplicationsImmunoprecipitation, Purification, Immunoprecipitation, Purification, Immunoprecipitation, Purification, Immunoprecipitation, Purification

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) The short-chain fatty acid crotonate reduces invasive growth and immune escape of Candida albicans by regulating hyphal gene expressionmBioNovember 6, 2023Christopher McCrory, Jiyoti Verma, Timothy M. Tucey, Rachael Turner, Harshini Weerasinghe, Traude H. Beilharz, Ana Travenincubated overnight at 4°C before addition of 20 µL of 50% suspension of protein A Sepharose beads (Abcam, ab193256) in lysis buffer and was incubated for 2 h at 4°C with shaking. The beads were pelleted and, following

(2) Umbilical cord mesenchymal stem cells relieve osteoarthritis in rats through immunoregulation and inhibition of chondrocyte apoptosisScientific ReportsSeptember 11, 2023Xin Pan, Xiongfeng Li, Ling Zhang, Feifei Wu, Qiang Zhang, Shasha Xu, Chengchun Shen, Jinfeng Liang, Ruolang Panantibodies overnight with stirring at 4 °C. After the addition of protein A-conjugated Sepharose beads (ab193256, Abcam, Cambridge, UK), the samples were incubated at 4 °C for 4 h and then centrifuged at 10,000×g

(3) VPRBP Functions Downstream of the Androgen Receptor and OGT to Restrict p53 Activation in Prostate CancerMolecular Cancer ResearchJuly 6, 2022Ninu Poulose, Nicholas Forsythe, Adam Polonski, Gemma Gregg, Sarah Maguire, Marc Fuchs, Sarah Minner, Guido Sauter, Simon S. McDade, Ian G. MillsStreptavidin Agarose Resin (20357) were obtained from Thermo Fisher Scientific. Protein A sepharose beads (ab193256) and protein G sepharose beads (ab193259) were from Abcam. Cell lines LNCaP and 22Rv1 cells were purchased

(4) Carbamazepine promotes surface expression of mutant Kir6.2-A28V ATP-sensitive potassium channels by modulating Golgi retention and autophagyJournal of Biological ChemistryMay 1, 2022Ching-Han Lin, Yu-Chi Lin, Shi-Bing Yang, Pei-Chun Chenco-IP, cell lysates were incubated with 2 μg of antibody and protein A-Sepharose beads (catalog no.: ab193256; Abcam) for 4 h at 4 °C. The immunocomplexes were then washed with triple lysis buffer three times and

(5) Immunization with synthetic SARS-CoV-2 S glycoprotein virus-like particles protects macaques from infectionCell Reports MedicineFebruary 15, 2022Guidenn Sulbaran et al.Kit,Macherey-Nagel,N/A ELISA microplates,Thermo Scientific,10547781 Protein A Sepharose ®,Abcam,Cat# ab193256 Protein G Sepharose ™,Abcam,Cat# ab17061801 Instant Blue Coomassie Protein Stain,Abcam,Cat# ab119211 Amicon®

(6) α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organizationeLifeFebruary 4, 2022Ma D, Wang F, Wang R, Hu Y, Chen Z, Huang N, Tian Y, Xia Y, Teng J, Chen J.for 15 min at 4 °C. Then, either Protein A-Sepharose beads or Protein G Sepharose beads (ab193259 and ab193256, respectively, Abcam, Cambridge, United Kingdom Amersham Biosciences) were added to the supernatants,

(7) BAD regulates mammary gland morphogenesis by 4E-BP1-mediated control of localized translation in mouse and human modelsNature communicationsMay 19, 2021Githaka JM, Tripathi N, Kirschenman R, Patel N, Pandya V, Kramer DA, Montpetit R, Zhu LF, Sonenberg N, Fahlman RP, et al.incubated with anti-BAD antibodies (1.5 h) and recovered with Protein A Sepharose beads (Abcam, Cat# ab193256). Western blotting Mammary gland lysates were generated as described for RPPA lysates, below. Mouse

(8) FGF8 and BMP2 mediated dynamic regulation of dental mesenchyme proliferation and differentiation via Lhx8/Suv39h1 complexJournal of cellular and molecular medicineMarch 1, 2021Zhou C, Chen D, Ren J, Huang D, Li R, Luo H, Guan C, Cao Y, Wang W.(ab172730; Abcam) for 12 hours at 4°C at a vertical shaking table. After that, 30 μL protein A Sepharose (ab193256; Abcam) was added for another 2 hours, followed by three washes with ice‐cold lysis buffer. The co‐immunoprecipitated

(9) The UPR sensor IRE1α and the adenovirus E3-19K glycoprotein sustain persistent and lytic infectionsNature CommunicationsDecember 1, 2020Vibhu Prasad, Maarit Suomalainen, Yllza Jasiqi, Silvio Hemmi, Patrick Hearing, Louise Hosie, Hans-Gerhard Burgert, Urs F. Greberkept for an input control sample and the rest was precleared with protein-A sepharose beads (Abcam, ab193256). Precleared lysates were incubated with anti-3A984, anti-Flag (Sigma, F7425), or an equivalent amount

(10) Exome Sequencing Identifies Genes and Gene Sets Contributing to Severe Childhood Obesity, Linking PHIP Variants to Repressed POMC TranscriptionCell metabolismJune 2, 2020Marenne G, Hendricks AE, Perdikari A, Bounds R, Payne F, Keogh JM, Lelliott CJ, Henning E, Pathan S, Ashford S, et al.protein (1mg/sample) and incubated with 20μl per sample of Protein Beads A (Protein A Sepharose, Abcam, ab193256) for 2 h at 4°C (pre-clean up). Samples were span at 800rpm for 30 s and incubated overnight with 5μl

(11) Dietary Restriction Extends Lifespan through Metabolic Regulation of Innate ImmunityCell MetabolismMay 1, 2019Ziyun Wu, Meltem Isik, Natalie Moroz, Michael J. Steinbaugh, Peng Zhang, T. Keith BlackwellProteins" Nystatin,Sigma,N1638 5-Fluoro-2’-deoxyuridine (FUDR),Thermo Fisher Scientific,AC227600025 TRI Reagent,Sigma,T9424 Protein A Sepharose,Abcam,ab193256 Isopropyl β-D-1-thiogalactopyranoside (IPTG),Fisher Scientific,BP1620–10 Lipopolysaccharides from Escherichia coli O111:B4

(12) MICU1 Interacts with the D-Ring of the MCU Pore to Control Its Ca2+ Flux and Sensitivity to Ru360Molecular CellNovember 1, 2018Melanie Paillard, György Csordás, Kai-Ting Huang, Peter Várnai, Suresh K. Joseph, György Hajnóczky,Life Technologies,L3000008 Complete protease inhibitor cocktail (EDTA free),Roche,11873580001 Protein A Sepharose,Abcam,ab193256 Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays DC Protein Assay,Biorad,5000112 Experimental Models

(13) Obese subcutaneous adipose tissue impairs human myogenesis, particularly in old skeletal muscle, via resistin-mediated activation of NFκBScientific reportsOctober 18, 2018O'Leary MF, Wallace GR, Davis ET, Murphy DP, Nicholson T, Bennett AJ, Tsintzas K, Jones SW.UK). Immunoprecipitation of resistin from adipose conditioned media 70 μL Protein A Sepharose® beads (Abcam, ab193256) were incubated with 1 μg anti-resistin primary antibody (polyclonal rabbit IgG, Thermo Fisher PA1-1049)

(14) Oxytocin Regulates Stress-Induced Crf Gene Transcription through CREB-Regulated Transcription Coactivator 3Journal of NeuroscienceSeptember 2, 2015Benjamin Jurek, David A Slattery, Yuichi Hiraoka, Ying Liu, Katsuhiko Nishimori, Greti Aguilera, Inga D Neumann, Erwin H van den Burg

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Protein A Sepharose from Abcam

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Protein A Sepharose from Abcam

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